The sub-project within the EU-funded Doctoral Network ViroiDoc focuses on the exogenous applications of RNA molecules for the non-transgenic control of resistance to viroid infections. Coding and non-coding regulatory RNAs are used to specifically modulate gene expression in plants. Various RNA species are used to strengthen the natural defense of plants against viroids.
The expansion of the RNA toolbox to include mRNA and antisense circulator RNA (caRNA) enables more precise gene regulations and intrinsic modifications, including the integration of CRISPR/Cas components. These innovative approaches not only improve the control of viroid infections, but also offer a wide range of applications in plant defense and sustainable agriculture.
The Wireless project develops innovative RNA-based strategies to control wireworms (Agriotes spp.), a major potato pest causing significant yield losses. Conventional chemical treatments are increasingly ineffective due to resistance and regulatory restrictions, highlighting the need for sustainable alternatives. The project uses RNAi to target essential wireworm genes with designed dsRNA molecules. These molecules will be tested in lab feeding trials and delivered in biodegradable carriers, such as starch-based capsules, which attract wireworms and protect the RNA until ingestion.
