RNA transport 

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The transport of RNAs within plants and between plants and pathogens plays a central role in plant-pathogen interactions and for RNA-based applications. Our research has shown that exogenous RNAs, including non-coding RNAs (ncRNAs), can be packaged into extracellular vesicles (EVs) that act as carriers for RNAi between plants and pathogens. This enables bidirectional communication, which we want to use specifically for the control of pathogens. 

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Particularly in the barley-fusarium system, we are investigating the role of EVs in the interorganismal, transport of RNAs. Our results so far indicate that EVs may play a more important role in biographic pathosystems than in necrotrophic ones. Using proteomics and RNA sequencing approaches, we identify key mechanisms that are crucial for the uptake and action of these vesicles. Furthermore, we analyze how extracellular RNAs cross the apoplast-symplast barrier though endo- and execytosis mechanisms.

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RNA processing

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One focus of our work is the processing of exogenous dsRNAs in plants. Using RNA sequencing and proteomic, we investigate how dsRNAs are cut by plant Dicer proteins and processed into effective siRNAs. we analyze which RNA-binding proteins such as Argonautes (AGO) stabilize these processed RNAs and influence their functionality.

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Our research provides important insights into the molecular basis of RNA transport and processing. These findings flow into the development of innovative RNA formulations with the aim of improving stability, efficiency and specificity. 

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By working closely with interdisciplinary networks and leading scientists, we are creating a solid foundation to revolutionize RNA-based technologies for sustainable plant protection. 

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